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DNA Sequence Analyzer

Paste a DNA sequence to instantly check its length, GC content, and find regions that might cause problems during synthesis or cloning. Think of it as a spell-checker for your gene.

Input Sequence
Enter your DNA sequence in FASTA format or as raw text. Valid characters: A, T, G, C

What You’ll Learn

What is GC content? It’s the percentage of bases that are G (guanine) or C (cytosine). This number matters because it affects:

  • How tightly DNA strands stick together (stability)
  • How well a gene gets expressed in cells
  • Whether PCR primers will work reliably
  • How easy or hard the sequence is to synthesize

Sliding window analysis: This tool scans your sequence in 30bp windows to flag spots where GC content is unusually high or low — the kind of trouble spots that can cause headaches in the lab.